self-organising map (som) profiling  (Partek)

Journal: bioRxiv

Article Title: Human skeletal muscle possesses both reversible proteomic signatures and a retained proteomic memory after repeated resistance training

doi: 10.1101/2024.11.19.624068

Figure Lengend Snippet: A , Venn diagram analysis of the statistically differentially regulated proteins: 1) RT1 vs. baseline, 2) DT vs. baseline, 3) RT2 vs. baseline, 4) DT vs. RT1 (pre-DT) and 5) RT2 vs. DT (pre-RT2) ( FDR < 0.05, FC >|1.2|). The 6th comparison: RT2 vs. RT1 is not shown as it did not produce any statistically significantly different proteins. The figure illustrates the number of common overlapping and unique significant proteins across each comparison. There were 285 proteins that were significantly different in at least one of the pairwise comparisons highlighted in the Venn diagram. B , SOM profiling of 285 proteins identified in the Venn diagram above in the PRT group over the 30 weeks time-course of baseline, RT1, DT and RT2.

Article Snippet: To plot temporal changes in proteins across the intervention, we undertook Self Organising Map (SOM) profiling of the change in standardised mean protein content within each time-point using Partek Genomics Suite as previously described earlier ( Turner et al ., 2020 ).

Techniques: Comparison

Journal: bioRxiv

Article Title: Human skeletal muscle possesses both reversible proteomic signatures and a retained proteomic memory after repeated resistance training

doi: 10.1101/2024.11.19.624068

Figure Lengend Snippet: A and B , Reactome pathway analysis together with Sankey-diagram illustrates that the retained proteins are enriched in cytokine signalling, biosynthesis, and folding, sorting and degradation pathways and functions. C , The 29 and 81 retained proteins (SOM) in GO have as a molecular function especially in calcium or calcium-dependent protein binding, actin filament binding and phospholipase inhibitor activity. D , CAPN2, CLIC1, MYH9 and CAPNS1 proteins increasing after RT1 and maintaining their levels after DT still above the baseline. N=17 in PRT and n=11 in CRT. * <0.05, ** <0.01 and *** <0.001 or exact FDR-value ( FDR < 0.10) vs. baseline. Data is presented as mean ± SEM.

Article Snippet: To plot temporal changes in proteins across the intervention, we undertook Self Organising Map (SOM) profiling of the change in standardised mean protein content within each time-point using Partek Genomics Suite as previously described earlier ( Turner et al ., 2020 ).

Techniques: Protein Binding, Binding Assay, Activity Assay